Propranolol Inhibits the Proliferation of Human Glioblastoma Cell Lines through Notch1 and Hes1 Signaling System

Objective : The anti-tumor effect of the beta-adrenergic receptor antagonist propranolol in breast cancer is well known; however, its activity glioblastoma not well-evaluated. Notch-Hes pathway known to regulate cell differentiation, proliferation, and apoptosis. We investigated human lines, role Notch Hes signaling this process.Methods performed immunohistochemical staining on 31 surgically resected primary tissues. also used lines U87-MG, LN229, neuroblastoma line SH-SY5Y study. isoproterenol proliferation was evaluated using MTT assay (absorbance 570 nm). impact gene expression (Notch Hes) real-time polymerase chain reaction (RT-PCR, whereas protein levels Notch1 Hes1 were measured Western blotting (WB), simultaneously. Small interfering RNA (siRNA) suppress investigate glioblastoma.Results Propranolol caused a dose-dependent decrease (MTT assay). RT-PCR showed an increase by propranolol, WB demonstrated protein, but propranolol. U87-MG LN229 significantly suppressed after transfection with siRNA.Conclusion These results that line, more closely involved than proliferation.